View source for Total Internal Reflection Fluorescence AFM

[[Category:Ambient AFM]]

{{EquipmentInfo
| name = TIRF-AFM
| Tool_Name = TIRF-AFM
| image = [[Image:EBL-01.jpeg|300px]]
| imagecaption = 
| Instrument_Type = AFM
| Staff_Manager = Matt Brukman
| Lab_Location = Room 014
| Tool_Manufacturer = Asylum / Nikon
| Tool_Model = MFP-3D
| NEMO_Designation = TIRF-AFM
| Lab_Phone = 8-9457
| SOP Link = [https://drive.google.com/file/d/1c9T_0DqiwEcC73Gxh6f4B_yrAiEq1GCT/view?usp=share_link SOP]
}}

== Description ==
The TIRF-AFM is an atomic force microscope with an 80μm in-plane scan range and 13μm vertical scan range that can operate in ambient atmosphere or fluid environment installed on an inverted optical microscope with total internal reflection optics and three laser lines of illumination. Its dedicated prep lab has a CO2 incubator and bio safety cabinet for sample storage and plating.
 
===== Applications =====
* Fluorescent imaging of cells, proteins, tissues, and nanoparticles
* Nano-injection of drugs and dyes
* Topographic imaging in contact and tapping (AC) modes
* Stiffness, friction, and adhesion measurements
* Piezoelectric force microscopy
* Conductive AFM (ORCA mode)
* Scanning Kelvin Force (surface potential)

==== Accessories ====
* Micromanipulator + nanoinjector system
* Photometrics Prime 95B camera 
* Nikon Eclipse Ti inverted optical microscope
* 633, 532, and 488 nm laser sources 
* Dual-gain ORCA tipholder
* High-voltage PFM tipholder
* Polymer heater sample holder
* Humidity-sensing sample holder
* Fluid heater sample holder (shared with TIRF-AFM)


== Resources ==

===== SOPs & Troubleshooting =====
* [https://drive.google.com/file/d/1c9T_0DqiwEcC73Gxh6f4B_yrAiEq1GCT/view?usp=share_link SOP]
* [https://upenn.box.com/s/5ihuen6v6cv1ocggg8zrg40uzophjxu6 Camera manual]
* [https://upenn.box.com/s/h9uz1oxocgi0cx1bxyv26c0h0k3dnh6p Inject Man Manual]
* [https://upenn.box.com/s/bkbkr4rlv3qutzjdiinvuz51ru61d3wg Femto Jet Manual]
* [https://upenn.box.com/s/0vlg3oezdnxkzetrxpq51gowg78tka4u Filter blocks]
* [https://upenn.box.com/s/xmrq89ittmwub0a1spg9irgx2t72jfvy Optics SOP]